Bestseller
Anti-Peptide mAb production
| Antigen | Price (without VAT, netto) **) |
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| anti-peptide antigen inclusive terminal cysteine residue. Host: 4 mice. |
Service features: Target protein analysis and peptide design Synthesis, purification & conjugation of your peptide (12-20 aa) Immunisation and sera collection ELISA identification Fusion and hybridoma development Isotype determination Ascites production and purification (2 clones) |
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Delivery: 50 ul pre-immune sera 2-5 mg purified monoclonal antibodies, mAb (2 clones) ELISA result - Titer guaranteed >1:50:000 Isotype determination result Project report 2 mg free peptide, purity >90% LC & MS analysis |
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Bestellnummer: JAB02-01DEZ23; Preis: 3.899 € |
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| **) Bei Angabe der USt.-ID Nummer ist der Angebotspreis auch gleich Rechnungsbetrag (netto = brutto). Zzgl. Versandkosten. Failure fee may apply. |
Peptidspezifische Affinitätschromatographie
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-Preparation of affinity column by covalent attachment of your peptide |
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-(your) antigenic peptide specific-Affinity chromatography of your antiserum |
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-Quantitation and ELISA of (your) specific antigenic peptide purified specific IgG antibodies |
| - Delivery: 2-5 mg purified specific IgG, Full report with ELISA data, guaranteed titer >1:40:000 |
IgY chicken antibodies
Chicken IgY Antibodies
In many instances, raising antibodies in chicken has proven essential. Where genetically distant species is required e.g. conserved mammalian proteins, Genosphere Biotechnologies will produce your antibodies in chicken. IgY antibodies may be isolated from circulating blood of chicken or extracted and purified in high yields from egg yolks of immunized laying hens.
Anti-peptide service offer:
• Synthesis, purification & KLH conjugation of your peptide
• Immunization of 2 hens
• Eggs collection, yolk isolation or antiserum collection and IgY purification
√ ELISA titered IgY antibodies fraction
√ Pre-immune purified IgY
√ Full report with ELISA analysis data
• 2 mg of unconjugated peptide (with HPLC & MS data, purity >90%)
Anti-protein service offer (you provide the antigen):
• Immunization of 2 hens
• Eggs collection, yolk isolation or antiserum collection and IgY purification
√ ELISA titered IgY antibodies fraction
√ Pre-immune purified IgY
√ Full report with ELISA analysis data
Pre-immune eggs are collected and control IgYs are purified. Following four immunizations over a 70-days period, eggs or antiserum are collected and we isolate the IgY fraction (purity 90-95%). The final antibodies preparation is then titered by ELISA. In addition, the total IgY fraction may be further purified by affinity chromatography using a peptide- or antigen-column to yield a specific hen antibody. For large scale IgY production, multiple hen immunization, and timely supply over long periods, please contact us with your project details and we will design the most efficient strategy for you.
Inquiries: info@genosphere-biotech.de
Fluorescent substrate for Furin protease
Specifications
Sequence: Pyr-Arg-Thr-Lys-Arg-AMC (AMC = 7-Amino-4-methylcoumarin).
Molecular Mass: 827.94 Da.
Purity: > 95% based on HPLC.
Quantity: 5 x 200 mg lyophilized powder
Price: Inquiry
Assay Conditions
A fluorescence plate reader with excitation at 380 nm and emission at 460 nm is recommended for
the measurement of the enzymatic activity. Depending upon individual enzymes, the substrate can
be used at final concentrations between 10 - 100 µM in a total of 100 µL reaction mixture.
Applications
Hydrolysis of Arg-AMC amide bond releases AMC, a highly fluorescent group.
It is an excellent substrate for furin-like proprotein convertases
MESH: Human Furin , West Nile Virus NS3 Protease
References:
K. Hatsuzawa, M. Nagahama, S. Takahashi, K. Takada, K. Murakami and K. Nakayama, J. Biol. Chem., 267, 16094 (1992)
Antimicrobial Peptides (AMP)
| Peptidname | L-AA-Sequenz; Gegenion: TFA, >80% Reinheit, Menge: 2 mg | Price (without VAT, netto) **) |
|---|---|---|
| Epinecidin-1 | GFIFHIIKGLFHAGKMIHGLV (21-mer) https://www.frontiersin.org/articles/10.3389/fmicb.2019.02631/full | 155,30 € |
| Sha | FLSGIVGMLGKLF (Two temporins, temporin-SHa and temporin-SHd, found in the skin secretions of the North African ranid frog Pelophylax saharicus, also target efficiently Gram-negative bacteria. However, with only 13 amino acid residues, temporin-SHa has a much broader spectrum, compared to temporin-SHd (17 amino acid residues), being potent against E. coli and A. baumannii, S. enterica serovar Enteritidis and K. pneumoniae.) | 149,90 € |
| **) Bei Angabe der USt.-ID Nummer ist der Angebotspreis auch gleich Rechnungsbetrag (netto = brutto). | Bitte fügen Sie bei Ihrer Bestellung die Peptidsequenz, Menge, Reinheit und Preisangabe einfach per copy + paste ein. |
Proteinexpression in Hefe (Yeast)
Service description
• Codon optimisation and gene synthesis
• Subcloning of gene in yeast expression vector
• Transform construct into E coli strain
• Prepare and linearise plasmid and transform 3-5 yeast strains (Hefe)
• Small scale expression, screening & expression pilot
• Scale up culture and purification
• Affinity purification (Ni2+, GST, etc)
• QC: SDS-PAGE analysis, western blot, purity, concentration
• Project report
Lead time: 9-10 weeks
Remarks:
Pichia pastoris expresses a very low amount of secreted proteins meaning the majority of proteins in the media represent the recombinant protein of interest. This property clearly simplifies the downstream protein purification process.
Pichia pastoris can be grown at high cell density. Thus,this host organism allows for high yield protein production.
Pichia pastoris possesses the complete post-translational machinery characteristic of eukaryotic cells. Thus, this host organism is able to perform phosphorylations and glycosylations (different from those observed in mammalian cells protein expression).
To conclude, Pichia pastoris protein expression is the first choice for the production of secreted proteins.
Isotopenmarkierte Peptide
Zugriffe heute: 4 - gesamt: 573.