Membranpotential & ROS Assay

JC-10 / ROS Assays

T-CA1310 Mitochondrial Membrane Potential Kit (JC-10 Assay) 130,00 € 100T

T-CA1410 Reactive Oxygen Species (ROS) Assay Kit 171,50 € 100T

Der JC-10-Assay ist eine empfindliche, fluoreszenzbasierte Methode zur Messung des mitochondrialen Membranpotentials , um Apoptose und Zellstress in lebenden Zellen zu überwachen. Im Vergleich zu JC-1 bietet JC-10 eine bessere Wasserlöslichkeit. Der Farbstoff akkumuliert in gesunden Mitochondrien (rot/orange) und diffundiert bei Apoptose als Monomer in das Cytosol (grün).

Microbial Viability Assay Kit (WST Method)

T-M3620 Microbial Activity Assay Kit (WST Method） 696,70 € 100T
store at 2-8℃

The Microbial Viability Assay Kit (WST Method) is a colorimetric, non-radioactive tool for rapidly quantifying viable microbes (bacteria/fungi) using water-soluble WST-8 salt. Metabolically active cells reduce WST-8 to an orange-colored formazan dye, with absorbance proportional to cell viability. It is commonly used for MIC (Minimum Inhibitory Concentration, e.g. antibiotics) determination.

Key Features and Principles:

Method: WST-8 is reduced to a soluble formazan dye by NADH/NADPH produced during microbial respiration (mediated by an intermediate electron acceptor).

Advantages: Unlike MTT, WST-8 produces a water-soluble dye, eliminating the need for organic solvent solubilization steps. It is less toxic to cells and allows for kinetic studies.

Application: Ideal for measuring bacterial cell viability, antibiotic susceptibility testing (MIC) against gram-positive and gram-negative bacteria, and antimicrobial screening.

Compatibility: Validated for various microorganisms including E. coli, S. aureus, and Candida species.

Limitations: Background absorbance can occur if samples contain high levels of reducing agents. The assay does not identify species, only total viable metabolic activity.

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