Proteinexpression in Säugerzellen

Mammalian cells

Service description

• Codon optimisation and gene synthesis
• Subcloning of gene in CMV promoter-based expression vectors
• Transform construct into E coli strain
• Prepare plasmid and transfect transient cells strains
• Screening for expression
• Scale up culture cells for transfection
• Affinity purification (Ni2+, GST, etc)
• QC: SDS-PAGE analysis, western blot, purity, concentration
• Project report

Lead time: 7-8 weeks

Where to start

You may contact us by email to: protein@genosphere-biotech.com

Please include the following information:

• Your gene or protein sequence or UniProt accession number

• Your preferred expression system(s)

• The mg amount of recombinant protein needed

• The purification level required: >80% or >90%

• Five expression systems
• Expression in bacteria Escherichia coli
• Expression in yeast Pichia pastoris
• Expression in baculovirus-infected insect cells
• Expression in HEK or CHO cells
• Expression in vitro (E. coli enzymatic transcription and translation machinery)

• Standard protein purity levels
Choice of purification levels required:
Purity >80 % or Purity > 90 %

• Affinity-purification tags
N- or C-terminal
Glutathione S-transferase fusion (GST tag)
6x or 10x histidine residues ((His)6 or (His10)tag)
etc…

• Fluorescent reporter proteins
As part of our recombinant protein expression services, we can design and produce proteins fused with fluorescent tags, including GFP, RFP, and mCherry. These fluorescent proteins are invaluable tools for monitoring expression, localization, and stability of recombinant constructs in real time.

Zugriffe heute: 5 - gesamt: 404.